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Cell cultures Cortical neurons in primary culture were prepared from fetal Wistar rats (E16), seeded at 2.5×10 5 cells per cm 2 in 6- or 12-well plates previously coated with poly-D-lysine (15 μg ml −1 ) in DMEM (Sigma, Madrid, Spain) supplemented with 10% (v/v) fetal calf serum (Roche Diagnostics, Heidelberg, Germany)

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Pretibial myxedema: pathophysiology and treatment options